In contrast to random primers, oligo (dT) primers will not be ideal for degraded samples but are superb for developing cDNA libraries and suggested for use with reverse transcriptases (RTs) as Superscript IV RT.
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Help The utmost number of candidate primer pairs to screen in an effort to obtain certain primer pairs (The applicant primers are generated by primer3 software).
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In contrast to oligo(dT) primers, these primers are not template precise and so are appropriate for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis making use of reverse transcriptase with mRNA templates.
In contrast to oligo(dT) primers, these primers aren't template precise and therefore are suitable for DNA synthesis making use of Klenow fragments with DNA templates or for cDNA synthesis utilizing reverse transcriptase with mRNA templates.
A maximum of 20 assembly accessions are authorized. FASTA sequences are limited to 300M. Be aware which the organism field is ignored for custom made databases. Enter sequence accession, FASTA sequence or assembly accession
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